Separation of proteins from human plasma by sample displacement chromatography in hydrophobic interaction mode

Fractionation and recovery of whey proteins by hydrophobic interaction chromatography.

A method for the recovery and fractionation of whey proteins from a whey protein concentrate (80%, w/w) by hydrophobic interaction chromatography is proposed. Standard proteins and WPC 80 dissolved in phosphate buffer with ammonium sulfate 1 M were loaded in a HiPrep Octyl Sepharose FF column coupled to a fast protein liquid chromatography (FPLC) system and eluted by decreasing the ionic streng...

Ampholyte-displacement chromatography for the separation of serum proteins.

The antibody fraction (less than 6ml) was added to immobilized enzyme (2ml of packed gel). The antibodies were recycled for 2h and the unattached antibodies washed from the gel for 1 h. Specific antibodies were eluted by washing with citric acid (0.1 M, pH2.2). Experiments marked with an asterisk were performed simultaneously. The molecular weights of immunoglobulin G and creatine kinase were t...

SEPARATION OF NONHISTONE HIGH MOBILITY GROUP (HMG) FROM HUMAN LYMPHOCYTES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

The high mobility group (HMG) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (HPLC). Reversed-phase HPLC under conditions of 50 mM triethylamine adjusted to pH 2.2 with phosphoric acid (solvent A) and 95% acetonitrile in water (solvent B) was used to separate proteins primarily on the basis of differences in the overall hydrophobi...

Hydrophobic Interaction Chromatography

Hydrophobic Interaction Chromatography

modeling for the design of a Biomimetic chimeric ligand. Application to the puriRcation of bovine heart L-lactate dehydrogenase. Biotechnology and Bioengineering 63: 321}331. Lowe CR (1984) Applications of reactive dyes in biotechnology. In: Wiseman A (ed.) Topics in Enzyme and Fermentation Biotechnology, vol. 9. Chichester: Ellis Horwood. Lowe CR, Burton S, Pearson J, Clonis YD and Stead CV (1...